Chronic ethanol feeding alters miRNA expression dynamics during liver regeneration.

BACKGROUND: Adaptation to chronic ethanol (EtOH) treatment of rats results in a changed functional state of the liver and greatly inhibits its regenerative ability, which may contribute to the progression of alcoholic liver disease. METHODS: In this study, we investigated the effect of chronic EtOH intake on hepatic microRNA (miRNA) expression in male Sprague-Dawley rats during the initial 24 hours of liver regeneration following 70% partial hepatectomy (PHx) using miRNA microarrays. miRNA expression during adaptation to EtOH was investigated using RT-qPCR. Nuclear factor kappa B (NFκB) binding at target miRNA promoters was investigated with chromatin immunoprecipitation. RESULTS: Unsupervised clustering of miRNA expression profiles suggested that miRNA expression was more affected by chronic EtOH feeding than by the acute challenge of liver regeneration after PHx. Several miRNAs that were significantly altered by chronic EtOH feeding, including miR-34a, miR-103, miR-107, and miR-122 have been reported to play a role in regulating hepatic metabolism and the onset of these miRNA changes occurred gradually during the time course of EtOH feeding. Chronic EtOH feeding also altered the dynamic miRNA profile during liver regeneration. Promoter analysis predicted a role for NFκB in the immediate-early miRNA response to PHx. NFκB binding at target miRNA promoters in the chronic EtOH-fed group was significantly altered and these changes directly correlated with the observed expression dynamics of the target miRNA. CONCLUSIONS: Chronic EtOH consumption alters the hepatic miRNA expression profile such that the response of the metabolism-associated miRNAs occurs during long-term adaptation to EtOH rather than as an acute transient response to EtOH metabolism. Additionally, the dynamic miRNA program during liver regeneration in response to PHx is altered in the chronically EtOH-fed liver and these differences reflect, in part, differences in miRNA expression between the EtOH-adapted and control livers at the baseline state prior to PHx

Resource interaction in smallholder farms is linked to farm sustainability: evidence from Indian Sundarbans

Efficient resource utilization in small-scale farms is crucial to achieving farm sustainability through endogenous mechanisms. However, the precise mechanisms to integrate farm resources to achieve farm sustainability are not very clear yet. By capturing the interaction among farm resources as a network phenomenon, we aimed to identify the discrete resource interactions (RIs) associated with higher farm sustainability in different farm types of Indian Sundarbans. First, we assessed the sustainability of 140 integrated farms using a synthesized assessment framework. Then, we considered four network motifs, namely linkage (a one-way link between two resources), reciprocal linkage (a two-way link between two resources), triad (three resources having closed interconnectedness), and the presence of a farm resource at the core of a network. Using RI network data of 140 farms and employing a graph theoretic approach we identified discrete network motifs (i.e., resource interaction) associated with highly sustainable farms in different farm types. We found a predominance of rice, vegetables and pond-based integration and identified 32 linkages, 11 reciprocal linkages, 21 triads, and three resources at the network core that occurred and co-occurred on highly sustainable farms, and thus critical to achieving farm sustainability. Further, multivariate analyses established that the properties of RI networks could explain farm sustainability significantly. We anticipate that sustainability in small-scale farms can be achieved by strategically designing new RIs on the farm. However, there may be limitations to such achievement depending on the nature of RI and the type of farm

Leydig cell tumor of testis in a child: An uncommon presentation

Leydig cell tumors (LCTs) are rare testicular tumors. Incidence is 1%–3% of all testicular neoplasms, bilateral in 10%. They are frequently hormonally active, leading to feminizing or virilizing syndromes. LCTs can be either pure or mixed with germ cell tumors or other sex cord-stromal tumors. Here, we are reporting a benign pure LCT in a 6-year-old boy presented with pseudopuberty

Scrotoschisis: An extremely rare congenital anomaly

A rare case of unilateral scrotoschisis in a full term infant delivered by Caesarean section is reported

LRRK2 gene G2019S mutation and SNPs [haplotypes] in subtypes of Parkinson\u27s disease

Mutation within the leucine-rich repeat kinase 2 (LRRK2) gene has been identified as a cause of autosomal dominant Parkinson\u27s disease (PD). The purpose of this study was to determine the frequency of G2019S mutation and whether the differences in the allele and genotype distribution of six SNPs within LRRK2 gene are associated with PD in an American non-Hispanic white population. The sample included 350 sporadic PD (SPD), 225 familial PD (FPD) patients and 186 controls of the same race and ethnicity. The frequency of LRRK2 G2019S mutation in our total sample of PD (FPD and SPD) was 1.56%. The frequency of this mutation was 3.5% in the FPD and 0.3% in the SPD groups, respectively. Allele and genotype frequencies of six SNPs were compared between PD and control samples. In addition, PD groups were categorized by sporadic PD (no family history), familial PD (first degree relative with PD) and age of onset (AON, or=51years). The haplotypes of the six SNPs were also constructed for association analysis. After correction for multiple comparisons, there was no association between any SNPs (allele or genotype) and PD groups. One of the haplotypes was modestly associated with the combined PD (SPD and FPD) sample. There was also no association with age at onset of PD. Our study suggests that the LRRK2 gene may be a risk factor or the cause for a very small fraction of PD in American white population

Ovarian cysts and tumors in infancy and childhood

Aim: Review of the clinical presentation, types (histology), and stages of presentation and overall outcome of ovarian tumors. Materials and Methods: This is a retrospective study. Forty nine girls from 3 days to 12 years were included in the study. Results: Fourteen girls had benign and thirty three had malignant ovarian tumors. One girl had bilateral ovarian non-Hodgkin lymphoma. Dysgerminoma (40%) was the commonest malignant tumor followed by malignant teratoma (16.6%). Conclusion: Pain and abdominal lump are the most common modes of presentation. Prognosis depends on the size of the tumor, stage and histology of the tumor. Conservative surgery should be the aim. Multidisciplinary management gives good prognosis